Re: Histamine Testing AOAC methode

From: Anthony Bimbo (apbimbo@verizon.net)
Date: Wed Oct 17 2007 - 13:01:23 PDT

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    Hello Dinesh:

    I just came across this and thought it might be of interest to you in the Maldives. I've done several FAO /Ministry of Fisheries projects related to the Male Market fish waste so this test kit might be of interest. I have no commercial relationship with Neogen but do have interest in histamine testing as related to fishmeal and the raw materials used to produce high quality fishmeal.

    Regards

    Tony Bimbo
    Technical Consultant
    International Fisheries Technology
    55 Cedar Lane, PO Box 1606
    Kilmarnock, VA 22482 USA
    Tel /Fax 804-435-3915
    Email apbimbo@verizon.net

    Newly approved histamine test for tuna

    Neogen Corporation has received approval from the AOAC Research Institute for its quantitative test for histamine that eliminates the need for the use of hazardous materials in the testing process, according to Neogen. Veratox for Histamine (AOAC-RI No. 070703) is a direct competitive ELISA test for the detection of histamine in fresh, canned or pouched tuna, packed in either oil or water. The test uses a uniquely simple water extraction process, and returns results in the range of 2.5 to 50 parts per million. The Veratox for Histamine AOAC validation studies showed a 95.4% recovery rate of spiked samples, when compared to the AOAC official fluorometric method, and no cross-reactivity to all other commonly occurring biogenic amines. www.neogen.com

      ----- Original Message -----
      From: Ensis Factory
      To: seafood@ucdavis.edu
      Sent: Sunday, October 07, 2007 12:42 AM
      Subject: Histamine Testing AOAC methode

      Dear Sea Food Listers,

      Any body have a Methode Validation for the Histaimne analysis using 5 grms fish weight and without keeping 65 Degrees C rather than use 10 gram and keep the homoginzed mixture at 65 degrees C (as it recommended by the AOAC offical methode.

      Looking forward for responses......

      Thanking you all

      Dinesh Dhammika,
      QA Manager
      Ensis Fishing Pvt Ltd
      Maldives



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