how to measure TMA in fish tissue by Dyer method

From: Shuming Ke (shumingk@foodsci.umass.edu)
Date: Fri Oct 05 2007 - 08:53:02 PDT

  • Next message: Jack Wekell: "Re: how to measure TMA in fish tissue by Dyer method"

    hi,
    I plan to use picrate method described by Dyer (j fish res bd can, 1945:351-
    358) to determine TMA content in marine fish tissue. The basic concept is to
    react TMA extracted in toluene with 0.02% picric acid and measure absorbance
    at 410nm. In Dyer' method, dry picric acid is disolved in toluene to prepare
    2% picric acid stock solution in toluene. Since dry picric acid is highly
    explosive and shock sensitive, this raises the question-- how to prepare
    picric acid solution in toluene? Do I just order dry (moisture free) picric
    acid solid and disolve in toluene? Any one has practical ideas on how to carry
    on the analysis? Many thanks. 

    -- 
Shuming Ke, Ph.D.
Research Associate
University of Massachusetts Marine Station
P.O.Box 7128
932 Washington Street
Gloucester, MA 01930
shumingk@foodsci.umass.edu

    Office: 978-281-1930 x. 2
Fax: 978-281-2618

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