Re: Bligh and Dyer Lipid Extraction Method

From: John C. Wekell (john.c.wekell@noaa.gov)
Date: Wed Aug 09 2006 - 09:17:21 PDT

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    Tony et al,
    I always look forward to Pete Howgate's replies! He has done a terrific summary of the state of the art. I would like to suggest an alternative, although not without problems, is to use methylene chloride (MC). We substituted MC for the chloroform many years ago and found that it worked quite well.  Our substitution was based on a variety of reasons, chief among them was its lower propensity for phosgene formation. However, the use of this solvent will be dependent on local regulations on chlorinated hydrocarbons and proper containment and disposal--these may not be insignificant for a particular operation/location. 

    A bit further afield but perhaps more current approach would be supercritical fluid extraction (SCF)  using carbon dioxide. SCF does require instrumentation and equipment that can be expensive but if  you are doing a large number of extractions, the cost can be amortized.

    Unfortunately, trying to find a substitute for the old B&D method is the old "between a rock and a hard place". Noxious solvents vs. expensive equipment, etc.


    --
    John C

    John C. Wekell, Ph.D.

    Research Chemist

    NOAA-Fisheries

    Environmental Conservation Division

    2725 Montlake Blvd. E.

    Seattle, WA 98112

     

    Work: (206) 860-3388

    FAX: (206) 860-3335

    http://www.nwfsc.noaa.gov/hab

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