Hello Everyone:
An issue just came up in a project I am doing for a client. The Bligh and Dyer Lipid Extraction Method has been around for a long time and is used to extract the total lipid from, among other things, fish tissue. The solvent mix is chloroform/methanol/water.
Chloroform is no longer allowed or is allowed under very controlled conditions so many methods that have used chloroform have switched over to some other solvent. For example in the AOCS method for Peroxide Value in oils and fats, the chloroform has been replaced by Isooctane. Collaborative tests have shown that the new solvent works fine.
I am wondering if anyone knows if the same has been done with the Bligh and Dyer Fat Extraction Method. Will Isooctane in conjunction with methanol and water work with the lipid extraction from fish tissue? If not, are there any suggestions?
Maybe someone up in Halifax NS can ask Graham Bligh about this.
Thanks
Tony Bimbo
Technical Consultant- International Fisheries
55 Cedar Lane, PO Box 1606
Kilmarnock, VA 22482 USA
Tel /Fax 804-435-3915
Email apbimbo@verizon.net
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