Mr. Ko:
In my opinion, your decription is the Conway method using Conway's chamber; when
you add the H3BO3 solution in the inner chamber you should close it
inmediatelly and hermetically with a sealed (i.g. w/vaseline) glass cover.
The reagents could change but, basically the technique you have described is
very well known and very usefull in practice.
Regards,
EB
Quoting "Ko, Iwan Setiyono" <isk025@student.uit.no>:
> Dear all,
> Can someone comments on this TVB-N method? Is this kinda modification of
> Conway's microdiffusion method? Or just using the Conway plate?
>
> ".... weigh 25 g sample, add 75 ml TCA 75%, and homogenize for 1 minute...
> take 1ml of the filtrate and put it into the left-outer chamber (plate?).
> Meanwhile, take 1 ml 2% H3BO3 and add it to the inner chamber, and 1 ml
> saturated (1:1) K2CO3 to the right-outer chamber... incubate at room
> temperature overnight or at 35 deg C for 2 hours....titrate with 0.02 N
> HCl until the boric acid turns from colorless (?) to pink...."
>
> Regards,
>
> Ko, Iwan Setiyono (Ko, I.S. - ISK)
> MSc Candidate - Int. Fisheries Management
>
> E-mail : isk025@student.uit.no
> Phone : +47 77 64 60 00
> Fax : +47 77 64 60 20
> Mobile : +47 45 00 40 97
>
> Visiting address:
> Room A-374
> Norwegian College of Fishery Science (NFH)
> University of Tromsų - Breivika N-9037 Tromsų
> NORWAY
>
>
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