Greetings all.
For your interest, we published hundreds of 'protein fingerprints'
obtained via electrophoresis from Australian (380 spp) and imported (175
spp) seafood species in 2 volumes of the Australian Seafood Handbook
(1999 and 2003). See http://www.publish.csiro.au/pid/3554.htm for more
details.
Warm regards,
Gus
______________________________
Gordon (Gus) Yearsley
CSIRO Marine Research
GPO Box 1538
(Castray Esplanade)
Hobart, TAS 7001, Australia
Phone: 03 6232 5144, 0432 620 253
Fax: 03 6232 5000
-----Original Message-----
From: owner-seafood@ucdavis.edu
[mailto:owner-seafood@ucdavis.edu] On Behalf Of Fred Stengard
Sent: Wednesday, 1 June 2005 5:25 AM
To: Emperor
Cc: seafood@ucdavis.edu
Subject: RE: Electrophoresus Testing for Finfish Species
Hi Robert,
I'm not sure if you got any responses to your query or not. Back
in grad school I used protein electrophoresis for looking into the
genetic relationships of the codfishes (order Gadiformes), and their
allies:
Stengard, F.J., 1998. Lactate Dehydrogenase Isozyme Patterns of
Paracanthopterygians (Osteichthyes: Teleostei): Evaluation of a Putative
Gadiform Synapomorphy, With Comments on Implications for Sister Group
Relationships and Phylogenetic Position of Gobiesociformes. Masters
Thesis, University of South Florida, St. Petersburg, Florida. 131 pp.
The best source that I found for protein electrophoresis info
that allows distinctions of fillets/muscle tissue of a wide variety of
food fishes is:
Shaklee, J.B. and C.P. Keenan. 1986. A Practical Laboratory
Guide to the Techniques and Methodology of Electrophoresis and its
Application to Fish Fillet Identification. CSIRO Marine Laboratories
Publ. 177. Melbourne, Australia.
It would probably include some circumtropical species common to
both Australian and Gulf of Mexico waters, but would not I believe
include non-Australian food fishes such as Red Grouper (Epinephelus
morio), Red Snapper (Lutjanus campechanus), and the like. I haven't kept
up well with the literature over the last seven or so years, so there
might be an equivalent Gulf of Mexico publication available now, but
there was none (as far as I'm aware) as of 1998.
Regarding your second question, I'm not sure how you mean
"professional capacity".. Are you getting at what expertise and
equipment would be necessary to perform such tests? Generally speaking,
you need quite a bit of specialized labware/equipment and chemicals
(some of which are rather expensive). Individual chemical recipes for
electrophoresis buffer systems, tissue grinding buffer, tracking dye,
histochemical staining solutions and buffer (to visualize the
end-products of the enzymatic reactions) starch gels, gel fixers, etc.
and directions for use must be more or less precisely followed.
Furthermore, interpretation of results is as much "art" as science, and
comes with experience - results are not always cut & dried. In short, it
is not a simple procedure that can be set up & utilized in your average
seafood company R&D lab.
Hope this helps.
Cheers,
Fredrik J. Stengard
Director of Research/QC/HACCP-Bio Safety
Bama Sea Products, Inc.
756 28th Street South
St. Petersburg, FL 33712
Phone (727) 327-3474 x374
Fax (727) 327-7574
email: fstengard@bamasea.com
-----Original Message-----
From: Emperor [mailto:emperor1@bellsouth.net]
Sent: Tuesday, May 24, 2005 2:02 PM
To: seafood@ucdavis.edu
Subject: Electrophoresus Testing for Finfish Species
Hello List,
Would any one know if a data base of genetic profiles
for Gulf of Mexico finfish exists anywhere as a result of
electrophoresus testing?
And,
Could someone tell me what kind of professional capacity
a person must possess to conduct and analyze electrophoresus testing;
especially relating to finfish proteins?
Thanks,
Robert Walker
Louisiana Seafood Exchange, Inc.
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