Hi Robert,
I'm not sure if you got any responses to your query or not. Back in grad school I used protein electrophoresis for looking into the genetic relationships of the codfishes (order Gadiformes), and their allies:
Stengard, F.J., 1998. Lactate Dehydrogenase Isozyme Patterns of Paracanthopterygians (Osteichthyes: Teleostei): Evaluation of a Putative Gadiform Synapomorphy, With Comments on Implications for Sister Group Relationships and Phylogenetic Position of Gobiesociformes. Masters Thesis, University of South Florida, St. Petersburg, Florida. 131 pp.
The best source that I found for protein electrophoresis info that allows distinctions of fillets/muscle tissue of a wide variety of food fishes is:
Shaklee, J.B. and C.P. Keenan. 1986. A Practical Laboratory Guide to the Techniques and Methodology of Electrophoresis and its Application to Fish Fillet Identification. CSIRO Marine Laboratories Publ. 177. Melbourne, Australia.
It would probably include some circumtropical species common to both Australian and Gulf of Mexico waters, but would not I believe include non-Australian food fishes such as Red Grouper (Epinephelus morio), Red Snapper (Lutjanus campechanus), and the like. I haven't kept up well with the literature over the last seven or so years, so there might be an equivalent Gulf of Mexico publication available now, but there was none (as far as I'm aware) as of 1998.
Regarding your second question, I'm not sure how you mean "professional capacity".. Are you getting at what expertise and equipment would be necessary to perform such tests? Generally speaking, you need quite a bit of specialized labware/equipment and chemicals (some of which are rather expensive). Individual chemical recipes for electrophoresis buffer systems, tissue grinding buffer, tracking dye, histochemical staining solutions and buffer (to visualize the end-products of the enzymatic reactions) starch gels, gel fixers, etc. and directions for use must be more or less precisely followed. Furthermore, interpretation of results is as much "art" as science, and comes with experience - results are not always cut & dried. In short, it is not a simple procedure that can be set up & utilized in your average seafood company R&D lab.
Hope this helps.
Cheers,
Fredrik J. Stengard
Director of Research/QC/HACCP-Bio Safety
Bama Sea Products, Inc.
756 28th Street South
St. Petersburg, FL 33712
Phone (727) 327-3474 x374
Fax (727) 327-7574
email: fstengard@bamasea.com
-----Original Message-----
From: Emperor [mailto:emperor1@bellsouth.net]
Sent: Tuesday, May 24, 2005 2:02 PM
To: seafood@ucdavis.edu
Subject: Electrophoresus Testing for Finfish Species
Hello List,
Would any one know if a data base of genetic profiles for Gulf of Mexico finfish exists anywhere as a result of electrophoresus testing?
And,
Could someone tell me what kind of professional capacity a person must possess to conduct and analyze electrophoresus testing; especially relating to finfish proteins?
Thanks,
Robert Walker
Louisiana Seafood Exchange, Inc.
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