Re: Comparison of Micro Results

From: Brian Himelbloom (ffbhh@uaf.edu)
Date: Thu Jul 01 2004 - 16:23:30 PDT

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    Hi Stella,

    I've encountered this situation before. From a purely mathematical
    standpoint, since 1 gram of water is equivalent to 1 ml and occupies 1
    cubic centimeter (cm3) of space, the conversion factor would be the
    square root of CFU/cm2 then cubed (or conversely the cube root of CFU/g
    then squared). However, I have not seen a peer-reviewed manuscript that
    has done the laboratory study to prove this conversion of three
    dimensions to two with regards to bacterial counts is valid
    statistically. Also, there are some other considerations beyond just
    the mathematics such as differences between the bacterial population
    surviving on a variety of food contact surfaces versus the bacterial
    population proliferating in the specific types of foods in question.
    Anyways, give it a go and see if the number crunching makes sense.

    Regards,

    Brian Himelbloom
    Assoc. Prof. Seafood Microbiology
    University of Alaska Fairbanks
    School of Fisheries and Ocean Sciences
    Fishery Industrial Technology Center
    118 Trident Way
    Kodiak, AK 99615-7401

    Stella Stacey wrote:

    >Dear List
    >
    >I am going to be validating a cleaning and sanitation programme and as
    >part of this will be taking contact slides (press plates) of dirty
    >surfaces prior to cleaning. The average of the dirty surface counts
    >(CFU/cm2) should not be greater than the expected (allowed) average APC
    >count for the product.
    >
    >I need some help with being able to make a comparison of a CFU/cm2
    >count and a APC/g count as I am sure there must be some conversion
    >factor when comparing results from a cm2 to /g.
    >
    >NOTE: These will be translated into log10 counts first.
    >
    >Thankyou for your assistance.
    >
    >Regards,
    >
    >Stella
    >
    >Stella Stacey
    >Quality Manager
    >Independent Fisheries Limited
    >Phone: +64 3384 2344
    >Fax: +64 3384 4650
    >E-mail: stella.stacey@indfish.co.nz
    >
    >



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