RE: Bacillus cereus in pasteurized crab

From: Richard Chivers (richardchivers@btconnect.com)
Date: Thu Jun 24 2004 - 02:56:05 PDT

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    Hi Ted,

    In ICMSF (1996) Micro-organisms in Food 5: Characteristics of Microbial
    Pathogens pp24-27 B cereus is described as being an aerobic, facultative
    anaerobic, as you say.

    It goes on to provide this data in the temperature range 85-129.4*C

    Medium
    pH
    Temp *C
    D (min)
    Z (*C)
    0.25 M phosphate
    7.0
    85
    33.8
    9.7
    0.25 M phosphate
    7.0
    85
    106.0
    13.9
    0.25 M phosphate
    7.0
    85
    34.4
    6.9
    0.06 M phosphate
    7.0
    95
    2.6
    10.0
    0.06 M phosphate
    7.0
    95
    21.7
    10.1
    Infant formula (milk based)
    6.3
    95
    2.7
    8.1
    Infant formula (milk based)
    6.3
    95
    15.3
    8.7
    Water
    -
    95
    36
    8.3
    Water
    -
    95
    36
    6.7
    Milk
    -
    95
    1.8
    9.4
    0.067 M phosphate buffer
    7.0
    115.6
    0.13-11.3
    7.9-9.9
    0.067 M phosphate buffer
    7.0
    121.1
    0.03-2.35
    7.9-9.9
    0.067 M phosphate buffer
    7.0
    126.7
    0.3
    7.9
    0.067 M phosphate buffer
    7.0
    129.4
    0.24
    7.9
    Distilled water
    -
    100
    5.5
    9.7
    Distilled water
    -
    115
    0.13
    9.7

    No further Z values given

    Hope this helps.

    Richard
    Seafood Audit International

    -----Original Message-----
    From: Ted Brillantes [mailto:tedb@jmbinter.com]
    Sent: Thursday, June 24, 2004 8:42 AM
    To: richardchivers@btconnect.com; HACCP mailing list (HACCP mailing list)
    Subject: RE: Bacillus cereus in pasteurized crab

    Hi Richard,

    Thank you for your reply. Yes, I did see this site but the D values are too
    wide, for example 21-137 minutes at 90 C.

    I also found the attached reference, but it also shows a very wide range --
    D85 at 33.8  106 min.
    By the way, the attached also shows that it can grow at anaerobic
    conditions.

    Any info on z value?

    Best regards,

    Ted

    -----Original Message-----
    From: Richard Chivers [mailto:richardchivers@btconnect.com]
    Sent: 24 June, 2004 2:09 PM
    To: Ted Brillantes; HACCP mailing list (HACCP mailing list)
    Subject: RE: Bacillus cereus in pasteurized crab

    Hi Ted

    Have you tried this for D values:

    http://seafood.ucdavis.edu/haccp/compendium/Chapt10.htm#Growth

    Perhaps USFDA chose C. bot as it is more common in a marine context than B.
    cereus. Also of course it is aerobic which will have a bearing on its
    development in can.

    Richard Chivers
    Seafood Audit International

    -----Original Message-----
    From: owner-seafood@ucdavis.edu [mailto:owner-seafood@ucdavis.edu]On Behalf
    Of Ted Brillantes
    Sent: 24 June 2004 06:36
    To: HACCP mailing list (HACCP mailing list)
    Subject: Bacillus cereus in pasteurized crab

    Dear List,

    I am reviewing HACCP for pasteurized canned crab.

    Could somebody tell me why USFDA picked up C. botulinum tye E and nonprot. B
    and F as the target microorganism for pasteurization instead of Bacillus
    cereus which seems to be more heat resistant ? (FDA Hazards and Control
    Guidance, Chap. 17: Pathogen Survival Through Pasteurization).

    Does anybody have information on B. cereus : D value, z value, etc?

    Thank you very much.

    Best regards,

    Ted Brillantes
    Quality Assurance Manager
    JMB International Ltd
    69 Soi Prasartsook, Yenarkard Rd
    Chongnonsee, Yannawa, Bangkok 10120
    Tel. (02) 249-1909, 249-1951, 249-1955, 671-0157 to 8
    Fax (02) 711-9341



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