Re: Histamines again!!

From: howgate (phowgate@clara.co.uk)
Date: Tue Oct 21 2003 - 05:16:53 PDT

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    Dear Ger

    I would draw your attention to the following paper:

    Nalan Gökolu, Changes in biogenic amines during maturation of sardine Sardina pilchardus marinade, Fisheries Science, 2003, 69, 823-829.

    The author used an HPLC method based on that of Mietz and Karmas.

    Peter Howgate

      ----- Original Message -----
      From: Ger Meade
      To: Seafood (E-mail)
      Sent: Friday, October 17, 2003 8:03 AM
      Subject: FW: Histamines again!!

      Subject: Histamines again!!

      Hello, yet another query on histamines, The HPLC method is that suggested by EU regulations for determination of histamines in Fresh, frozen and salted Herring. I have been trying to find out what effect, if any, the acetic acid:brine mix used in marinades would have on this method of determining histamines? Should there not be some adjustment for pH? I would appreciate any assistance on this matter. I have been comparing kits to hplc method and am having difficulty with the results in marinades.
      With Best Regards

            Ger Meade
            Company Biologist / Quality Control Manager
           ) + 353 73 31004
            3 + 353 73 31509
            * ger@gallagherbros.ie
           



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