Attached is a copy of the procedure we are using in our laboratory for the histamine analysis - fluorometric method, though i would like to suggest that you refer to the original source for reference (AOAC, 1990) or an updated version. I hope this could help.
Dear Members,
My sincerest gratittude for your comments and suggestions. More power.
Sincerely,
GRACE N. PALMOS
University Research Associate I
Institute of Fish Processing Technology
College of Fisheries and Ocean Sciences
University of the Philippines in the Visayas
5023 Miag-ao, Iloilo, The Philippines
Telefax No.: (+6333) 3159285
Mobile No.: +639174676259/+639186561013
>From: "BAD"
>To: Rogério Mendes , "'GRACE PALMOS'"
>CC:
>Subject: RE: histamine on non-scombroid species
>Date: Wed, 19 Feb 2003 07:41:28 -0500
>
>Dear members:
>
>I have been receiving your information on different topics, which I do
>considering very important and usefull..I do it appreciate very much…
>
>I am the owner of a Laboratory in Colombia, and one of the topics, which, we
>will like setting up is the HISTAMINE analysis, fluorometric method… I would
>like requesting the protocol for the fluorometric method if possible and
>particularly the type of filters for the fluorometer for this specific
>methodology..
>
>Additional to that, we recently had adquired a GC/MS from THERMOFINNIGA and
>we would like to set up methodology for BIOGENIC AMINES in particularly
>CADAVERIN AND PUTRESCIN.. do some of you know about this methodology in
>particularly??…do this methodology wiilll include in this case the
>HISTAMINE..
>
>Thanks a lot for your kindly cooperation and answer
>
>
>BELISARIO ACEVEDO
>Ph.D FOOD CANNING
>ASINAL LTDA
>BOGOTA
>COLOMBIA
>
>-----Original Message-----
>From: owner-seafood@ucdavis.edu [mailto:owner-seafood@ucdavis.edu]On Behalf
>Of Rogério Mendes
>Sent: Miércoles, 19 de Febrero de 2003 03:56 a.m.
>To: 'GRACE PALMOS'
>Cc: seafood@ucdavis.edu
>Subject: RE: histamine on non-scombroid species
>
>This is a good method with postcolumn derivatization for determination of
>all biogenic amines.
>
>Liquid chromatographic method for determination of biogenic amines in fish
>and fish products.
>Veciana-Nogues-MT; Hernandez-Jover-T; Marine-Font-A; Vidal-Carou-M-del-C
>1995
>Journal-of-AOAC-International; 78 (4) 1045-1050, 23 ref.
>
>Best regards
>
>Rogério Mendes
>Department of Technological Innovation and Upgrading of Fish Products
>National Institute of Agrarian and Fisheries Research - INIAP
>Av. Brasília
>1449-006 Lisboa
>PORTUGAL
>Phone: ++351 21 3027036/21 3027000
>Fax: ++351 21 3015948
>Internet: http://ipimar-iniap.ipimar.pt
>
>
>
>-----Mensagem original-----
>De: GRACE PALMOS [mailto:grazia1377@msn.com]
>Enviada: terça-feira, 18 de Fevereiro de 2003 7:22
>Para: Assunto: histamine on non-scombroid species
>Dear List,
>A good day to you all. It's my first time to post, having been recently
>accepted to join the group. I'm a research assistant in our university and
>i hope you can help me on our problem. We are surrently working on the
>presence of histamine on some non-scombroid species of fish abundant in our
>area (sardines and bangus/milkfish). Sardines is primarily dried and sold in
>the market and the milkfish is used to produce value-added products
>(deboned, smoked, surimi-based products, etc.). I would like to inquire if
>there is a method of histamine analysis which is specific for dried
>products. We are currently using the AOAC method (fluorometric), but just
>recently we had acquired 1 unit of HPLC(it's quite difficult to ask for
>research fundings due to the limited budget allocated by the government),
>thus anyone has a method for Histamine Analysis using HPLC or could you
>refer me to some references where we could get a method.
>My gratitude for your comments and suggestions.
>GRACE N. PALMOS
>Institute of Fish Processing Tehnology
>College of Fisheries and Ocean Sciences
>University of the Philippines in the Visayas
>5023 Miag-ao, Iloilo
>The Philippines
>E-mail: grazia1377@msn.com /
>grazia1377@yahoo.com
>
>
>
> _____
>
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