Hello Kathleen: IFFO, the Int. Fishmeal and Fish Oil Organization
conducted a ring test during 2001 on the determination of histamine in
fishmeal. Labs used the AOAC fluorometric method, HPLC or test kits. When
I asked for comments from the participants on the protocol, no one remarked
that histamine sticking to glassware was an issue. There were 2 kit
manufacturers involved in this ring test and all of the laboratories were
"expert" in this analysis in that they were internationally recognized and
approved commercial labs. The only mention for HPLC is that the glassware
must be thoroughly cleaned and suitable for HPLC analysis.
Hope this helps.
Regards
Tony Bimbo
----- Original Message -----
From: "Barber, Kathleen G. (BB)" <barberk@bumblebee.com>
To: <seafood@ucdavis.edu>
Sent: Tuesday, February 11, 2003 3:50 PM
Subject: FW: Use of Glassware for Histamine Analyses
> -----Original Message-----
> From: Barber, Kathleen G. (BB)
> Sent: Monday, February 10, 2003 3:34 PM
> To: 'seafood@ucdavis.edu'
> Subject: Use of Glassware for Histamine Analyses
>
> Can anyone enlighten me on the use of glassware for the extraction step
> when testing for histamine? One of the screening test kits says that
> histamine may adhere to glass and I do recall some mention of this many
> years ago, but I could not find any reference to not using glassware in
> the extraction step for the fluorometric method (AOAC 16th edition,
> 35.1.32).
>
> Thanks.
>
> Kathleen Barber
This archive was generated by hypermail 2b29 : Tue Feb 11 2003 - 10:22:30 PST