Student needing help!!!

From: D. Grey Allen (dgallen2@unity.ncsu.edu)
Date: Wed Feb 05 2003 - 14:59:04 PST

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I am a student at NC State University, and I am looking into inoculating a sterilized (autoclaved) fish tissue based media (either tuna and/or mahi-mahi) with bacterial isolates and determining the rate they produce volatile basic nitrogens. My question is, would there be enough TMAO and other substrates left, after autoclaving, to interact with bacterial enzymes to produce volatile basic nitrogens. Any references that illustrate the answer to this question would be appreciated.

Thank you,
D. Grey Allen

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