Dear Jerry:
Unfortunately the people who is in charge to test
shrimp samples and certificate them as
"chloramphenicol free" said that detection limit for
ELISA is 0 (zero). Can you imagine that? And they
are "the official organisms of control".
Worst than that, they don´t know how to explain cross
reactions incidence (0.01%). When I ask them what is
that mean, they said at first: ...if you have 10,000
ppm of florfenicol in your sample, ELISA
chloramphenicol will detect 10,000 X 0.01% = 1 ppm.
After they said from n samples with florfenicol, the
possibility of detection is 0.01%.
Please, can you tell me more about the GC-ECD method
published in JAOAC by them for all samples and GC-MS.
Where can I find this reference?
Gabriel Rivera
--- "E. Jerry Oliveras, Laboratory Director &
President" <jerry@anresco.com> escribió: > The
detection Limit of any analysis cannot be ZERO
> this is a physical impossibility.
>
> I would sincerely question the technical expertise
> of anyone claiming a ZERO
> detection limit for an analysis.
>
> cross reactivity is an essentially unrelated
> parameter of specificity
>
> tests can be highly sensitive [very low detection
> limits] but have poor
> specificity and the converse etc.
>
> Also I have been told by the FDA lab staff in Denver
> that they are not using
> screening tests but are going straight to the GC-ECD
> method published in
> JAOAC by them for all samples - then confirming any
> positives by GC-MS.
>
> Jerry
> Laboratory Director
> ANRESCO
>
> -----Original Message-----
> From: Gabriel Rivera [mailto:griveralo@yahoo.com]
> Sent: Wednesday, February 20, 2002 7:36 AM
> To: Daniel_Núñez_T.; seafood@ucdavis.edu
> Subject: Re: Chloramphenicol rapid test
>
>
> Daniel:
>
> Here in Ecuador there are some institutions (SGS,
> INP,
> etc.) which are doing ELISA tests for
> chloramphenicol.
> This is the standard method accepted by FDA as
> first
> method of chloramphenicol detection. If this method
> give a postive result, then they check by HPLC to
> confirm.
>
> They say the detection limit for ELISA is 0 (zero),
> but there is an incidence percentage, 0.01%, for
> cross
> reactions with other antibiotics of the same family
> (tiamphenicol and florfenicol).
>
>
> --- "Daniel_Núñez_T." <daniel@sudamar.com>
> escribió:
> > Dear list members:
> >
> > Does anyone know the reliability of the rapid
> tests
> > kits for chloramphenicol detection in shrimp
> > (aquaculture)?
> >
> > I found one in internet site of the AOAC:
> >
> > Chloramphenicol r-Biopharm GmbH / Bio-Tek
> > Instruments, Inc. in USA RIDASCREEN
> Chloramphenicol
> > milk, meat, eggs, media
> >
> >
> > Does anyone hear about their results with these
> kind
> > of tests?
> >
> > Are there more companies that work with these kind
> > of tests?
> >
> > Thanks in advance for your cooperation.
> >
> > Best regards,
> >
> > Eng. Daniel Núñez T.
> > ASQ Certified Quality Auditor - HACCP Lead Auditor
> > Sudamar Technical Manager - Seafood Quality
> > Assurance Company
> >
> >
>
>
_________________________________________________________
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>
>
=====
Ac. Gabriel Rivera L.
Director de Investigación y Desarrollo - FARMAVET
Alborada Etapa 11, Mz. 44, Villa 10, Av. Benjamín Carrión y Av. Isidro Ayora, Guayaquil-Ecuador
Telf. 593-4-2241510/2239575/2239719/2239734/2238798/2279808
Fax 593-4-2243936
Celular 593-9-9989311
Fax personal 1-435-508-3414 (USA)
_________________________________________________________
Do You Yahoo!?
Información de Estados Unidos y América Latina, en Yahoo! Noticias.
Visítanos en http://noticias.espanol.yahoo.com
This archive was generated by hypermail 2b29 : Wed Feb 20 2002 - 12:44:38 PST