Dear Alan:
As you may be aware of, there is quite a good inverse correlation (often
r=0.99) between fat and moisture. Of course, this is specific to each
species. Since you are interested in estimation, measuring the moisture
content of the well represented homogenate of fish tissue will be a quick
solution. First, you need to establish such a correlation with a linear
function from which you can convert moisture value to fat content. The best
way is to aquire data during a particular harvest season, or if the season
runs yearround, you may need to collect data throughout the year. We also
have developed a solvent extraction method which you may need a solvent as
little as 20 ml (chloroform + methanol) for 2 g sample (AOAC Intern. 79(2):
487 (1996). In this report, we used 50 ml to 5 g. It takes a little over
an hour. But you need a good balance. I'll be happy to go in detail, if you
are interested in.
Chong
Chong M. Lee, Professor
Dept. of Food Science & Nutrition
University of Rhode Island
Kingston, RI 02881
Tel. 401-874-2862; Fax. 401-874-2994
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